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This study proposed a model based fault detection and isolation (FDI) method using multi-layer perceptron (MLP) neural network. Detection and isolation of realistic faults of an industrial gas turbine engine in steady-state conditions is mainly centered. A bank of MLP models which are obtained by nonlinear dynamic system identification is used to generate the residuals, and also simple thresholding is used for the intend of fault detection while another MLP neural network is employed to isolate the faults. The proposed FDI method was tested on a single-shaft industrial gas turbine prototype and it have been evaluated using non-linear simulations based on the real gas turbine data. A brief comparative study with other related works in the literature on this gas turbine benchmark is also provided to show the benefits of proposed FDI method.