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The study is to validate the transmission route of rabbit meridian and the organizational structure of meridian in cellular level. The water solution of Na2H32PO4 (18.5MBq/0.25ml) was injected into “waiguan”, “neiguan”, “yang gu”, “taixi” acupoint according to human meridian. The transmission route and the tissue structure of rabbit meridian were detected with 32P whole and light microscopic autoradiography. The results indicated that the whole autoradiograms of rabbit meridian showed more wide and thick line than that of human. A great quantity of 32P was found in the stratum of epidermis and the tactile corpuscle, which is also same as human studies. The results reveal that the rabbit body surface existed low resistance of acupoints. The transmission route of rabbit meridian is closely related to the brain, spinal sensory free nerve endings and cutaneous nerve. We can try to research the material basis meridians sensation conduction by the animal model.