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This paper introduces the functions and characteristics of Multisim software, and describes the new methods of using the software in digital circuit experiment teaching. Examples about the experiment using Multisim are given in detail from three aspects which are the basic experiment, the advanced experiment and the integrated experiment. It believes that using Multisim in experiment teaching makes effective complementarities to the traditional experiment. This new method plays an important role in improving experiment teaching effection, exploding student's interest and cultivating innovative ability.