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In order to observe the effect of fluoride and arsenite on vascular endothelial cells and to explore their alone or associated mechanism on the blood circulatory system. Rat aorta endothelial cells was cultured in Vitro and was contaminated with NaF at 0μmol / L, 600μmol / L and 900μmol / L and NaAsO2 0μmol / L, 0.1μmol / L, 1.0μmol / L compatibility for 48h. Cell morphology was observed with light microscopy, cell activity was detected by MTT, apoptosis was by flow cytometry, DNA damage in endothelial cells was analyzed through single cell gel electrophoresis. The cells of non-exposed control group and the low concentration of fluoride and arsenite group grew well and showed pebble-shaped spindle-shaped, but they appeared poor growth state, some were round with necrosis in alone or associated with high concentrations groups. The cell activity and DNA damage in fluoride and arsenite alone or associated with high concentrations groups was lower and more seriously than in non-exposed control group and low concentration group (P<;0.05), and the cells apoptosis was the opposite result. Fluoride and arsenite alone or in combination can reduce the activity of vascular endothelial cells, induced apoptosis, induce DNA damage, and there was a dose - response relationship.