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Objective: To establish an accurate, rapid and efficient method to identify the characterization of DNA fingerprint from Trionycis carapax by using molecular biology techniques. Methods: Mitochondrial DNA (mtDNA)was extracted from Trionycis carapax by phenol extraction method and salting-out method respectively. The cytobchrome b (Cytb) partial gene from Trionycis carapax was amplified with PCR by designing specific primers and the PCR products were sequenced and blasted in Genbank. Results: Complete 17.3 kb mtDNA was extracted from Trionycis carapax. The Cyt b gene sequence revealed that the 362 bp segments were identical to that described in GenBank accession number AY687385. Conclusion: Molecular biology techniques can be applied to identify the characterization of DNA fingerprint from Trionycis carapax, with achieving the efforts that were rapid, efficient in addition to high specificity.
Date of Conference: 19-22 Aug. 2011