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In the present work, the microfabricated impedance sensing array (MISA) unit, based on interdigitated microelectrodes (IDMEs) coated with nickel hexacyanoferrate (NiHCF) film, is presented as an alternative solution to detect pathogens. The NiHCF film, which provide a [Fe(CN)6]3-/4- probe-free redox reaction, was functionalized with hexahistine tags (6×His) antibodies with which they have the high-affinity. Variation in the interfacial impedance caused by bio-activated beads was measured to detect targeted Infectious Salmon Anaemia Virus (ISAV) antigen. A detection limit of 10pg/ml was demonstrated, which indicates that the MISA unit is promising to detect pathogens at early-stage of the infection.