By Topic

Rapid isolation and detection of methicillin-resistant Staphylococcus aureus by using a microfluidic system

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$31 $13
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

5 Author(s)
Yu-Hsin Liu ; Dept. of Eng. Sci., Nat. Cheng Kung Univ., Tainan, Taiwan ; Kang-Yi Lien ; Chih-Hung Wang ; Jiunn-Jong Wu
more authors

This paper presents an integrated microfluidic system for rapid detection of methicillin-resistant Staphylococcus aureus (MRSA) by utilizing antibody-conjugated magnetic beads. By using immunomagnetic beads conjugated with specific antibodies and on-chip nucleic acid amplification, the developed microfluidic system can detect MRSA bacteria with a high sensitivity and specificity when compared with the bench-top diagnostic systems. The MRSA can be firstly bound onto the surface of the magnetic beads, followed by purifying the magnetic complexes with the incorporation of permanent magnet and the microfluidic control module. Finally, the specific genes of MRSA can be amplified by utilizing an on-chip polymerase chain reaction (PCR) module. Hence, the integrated microfluidic system can automatically perform isolation and detection of MRSA within a short period of time and can be used as a promising platform for biological and medical applications in the future.

Published in:

Nano/Micro Engineered and Molecular Systems (NEMS), 2011 IEEE International Conference on

Date of Conference:

20-23 Feb. 2011