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This paper introduces a kind of high-speed real-time information collection system based on FPGA and USB. Through the software and hardware technology, the system uses the combination of EP2C8Q208C8FPGA chip of Cyclone II series, FT245BM type fast USB communication interface chip of FTDI company and HK-2000 G pulse transducer. The system realizes the collection of pulse signal, and the real-time data's storage and the show of pulse wave on PC. According to the change of the pulse condition, doctors will know better about the health condition of human body and it's beneficial to analysis the cause.