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In order to collect the radio frequency (RF) signal in high speed, a RF data acquisition system based on STM32 and FPGA is designed in this paper. The RF signal received from the antenna is processed by conditioner circuit, high speed ADC, FPGA and STM32 and transmitted to the computer through USB. The serial peripheral interface (SPI) with direct memory access (DMA) technology is used to ensure the communication in high speed between STM32 and FPGA. The system test indicates that the system can acquire data with high precision and high speed.