Cart (Loading....) | Create Account
Close category search window

Plasma-cell-interaction: Expression of surface molecules on HaCaT keratinocytes after treatment with dielectric barrier discharge (DBD) plasma

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$31 $13
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

6 Author(s)
Haertel, B. ; Univ. of Greifswald, Greifswald, Germany ; Lindequist, U. ; Haehnel, M. ; Wende, K.
more authors

Summary form only given. Non-thermal atmospheric-pressure plasmas have been developed that will be used for several indications, e.g. also in medicine. Here, living tissues and cells are in focus of plasma treatment, e.g. to improve wound healing or to induce apoptosis and growth arrest in tumour cells. Detailed investigations of plasma-cell interactions are strongly needed. The cell membrane with its embedded proteins is the first target of plasma treatment. Such cell surface molecules as integrins, cadherins or the epidermal growth factor receptor (EGFR) are of importance in wound healing and also for development of cancer metastasis. This study focused on measurement of cell surface molecules on human HaCaT keratinocytes promoting adhesion, migration and proliferation as one important feature of plasma-cell interactions. HaCaT keratinocytes were treated with plasma by a surface dielectric barrier discharge in air. Cell surface molecules were analyzed by flow cytometry 24h after plasma treatment. Besides a reduction of cell viability a significant down regulation of E-cadherin and the EGFR expression was observed. The influence on α2- and β1-integrin was less pronounced and expression of ICAM-1 was not affected. The extent of effects depended on the treatment regimen and the exposure time of cells to the plasma. The two-dimensional culture of HaCaT keratinocytes are proved to be a suitable method of investigating plasma-cell interactions.

Published in:

Plasma Science (ICOPS), 2011 Abstracts IEEE International Conference on

Date of Conference:

26-30 June 2011

Need Help?

IEEE Advancing Technology for Humanity About IEEE Xplore | Contact | Help | Terms of Use | Nondiscrimination Policy | Site Map | Privacy & Opting Out of Cookies

A not-for-profit organization, IEEE is the world's largest professional association for the advancement of technology.
© Copyright 2014 IEEE - All rights reserved. Use of this web site signifies your agreement to the terms and conditions.