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Wireless mesh networks (WMNs) have attracted much attention lately for providing efficiently wireless services with high quality of service (QoS). Metropolitan WMNs are a low-cost solution for providing broadband wireless internet access in large areas. One of the fundamental issues of wireless communications is interference. In WMNs interference can be caused by simultaneous transmissions at links internal to the mesh network or by external sources. In this work we perform extensive measurements in a multi-radio metropolitan WMN deployed in the city of Heraklion, Greece. The basic goal is to investigate the impact of interference on the performance of the multi-path WMN. Towards that goal, we perform measurements with FTP, video streaming and raw data traffic for two scenarios, one with an efficient channel assignment (CA) that accounts for interference and another with a random CA that results in high internal and external interference in the network. The results prove that interference creates severe performance degradation, with regards to high delay, high packet losses, low throughput and low signal-to-interference and noise ratio (SINR). As a result, the metropolitan WMN becomes unable to support multi-path flows and demanding applications with an acceptable QoS.