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Here, we report an experimental realization of optimal phase-covariant quantum cloning machine with a single electron spin in solid state system at room temperature. The involved three states of two logic qubits are encoded physically in three levels of a single electron spin with two Zeeman sub-levels at a nitrogen-vacancy defect center in diamond. The preparation of input state and the phase-covariant quantum cloning transformation is controlled by two independent microwave fields. The average experimental fidelity reaches 85.2% which is very close to theoretical optimal fidelity 85.4% and is beyond the bound 83.3% of universal cloning.