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This research discussed about grid environment by using Globus Toolkit and alchemi, it is part of grid environment in Middleware layer where use it for operate grid application, controlling resource, and can distribute with various geographic, also support parallel application like MPI (message Passing Interface). Globus Toolkit uses component of MPICH-G2 to execute mpi application. Globus used authentication for users, data management. Resource and data security, globus operates in linux system operation. And for alchemi operates in windows environment and it is support framework architecture NET. This research to evaluate performance Globus Toolkit and Alchemi by made desain an environment for mpi application. Experiment has done by some parameter to analyze result. Hopefully, this final research can be comparation for build grid environment.