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Stand-alone micro fluidic system using partly disposable PDMS microwell array for high throughput cell analysis

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4 Author(s)
Kasumi Miyamoto ; Major in Nano-Science and Nano-Engineering, Waseda University, Japan ; Ryo Yamamoto ; Kentaro Kawai ; Shuichi Shoji

A stand-alone microfluidic system consists of a reusable flow control part and a disposable microwell array for high throughput cell analysis is proposed. The flow control part is realized with addressable microvalve array driven by compactly-fabricated solenoid actuators and EOF micropumps. Disposable 16 microwell array with 4 reagent injection ports was fabricated using a PDMS structure. The disposable fluidic chip can be easily replaced to different functional fluidic networks depended on their actual uses. The prototype system with 25 (52) on-chip hydraulic valves that are driven by 10 (2×5) solenoid actuators was fabricated. Precise reagent injection of ten-nanoliter order to each microwell was successfully carried out.

Published in:

2011 16th International Solid-State Sensors, Actuators and Microsystems Conference

Date of Conference:

5-9 June 2011