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Specific cell capture and temperature-mediated release using surface-immobilized aptamers in a microfluidic device

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5 Author(s)
Jing Zhu ; Dept. of Mech. Eng., Columbia Univ., New York, NY, USA ; ThaiHuu Nguyen ; Renjun Pei ; Stojanovic, M.
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We present a microfluidic device with an integrated temperature sensor and heaters for capture as well as subsequent temperature mediated release and recovery of CCRF-CEM cells. Specific cell capture is achieved by a surface immobilized aptamer. A moderate temperature change is then introduced to disrupt the cell-aptamer interaction, allowing the release of captured cells from the aptamer modified surface and their retrieval for further analysis. Experimental results demonstrate that the device is capable of handling a wide range of cell concentrations, and that the released cells remain viable.

Published in:

Solid-State Sensors, Actuators and Microsystems Conference (TRANSDUCERS), 2011 16th International

Date of Conference:

5-9 June 2011