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The adaptive genetic algorithm (AGA) designed as a general optimization method was applied to the ambiguity resolution of pulse Doppler (PD) radar. The fitness, based on squared error for multi-PRF (pulse repeat frequency) consecutive ordered ranges, was designed. The crossover operator and the conditions of ending in GA were discussed. The relations among the probability of ambiguity resolution of this algorithm, the measurement error and the computational efficiency were analyzed. Simulation results show the GA ambiguity resolution algorithm does better than the sliding window algorithm on the probability of ambiguity resolution.