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A physical fitness condition measurement system based on radio frequency identification (RFID) technology is proposed in this paper. The system includes a RFID tag, a RFID reader, a main control board, and several peripherals such as blood pressure meter, ear-temperature meter, height-weight meter, balance measurement, and speed test for running back-forth measurement. The RFID tag and reader are used to store and read a user's identification as well as their physical fitness conditions. The main control board with MINI2440 ARM microprocessor and WinCE 6.0 platform embedded would process the user's identification and trigger the peripheral device for measuring his/her physical fitness conditions. The measured data is then transmitted and stored in the RFID tag and database. The user interface and database are built by C++ codes. The proposed system with easy operations has been tested by a blood pressure meter, an ear-temperature meter, height-weight meter, speed test, and so on. According to the experimental results, the outputs of the measured data coupled with users' body mass index (BMI) can be used as self health management references and support medical diagnosis to quantify the medical treatment, rehabilitation or training.