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Evaluation of human surface muscle activity and stress, such as face muscles, is important for the functional study, diagnosis, prosthetic and orthodontic therapy. The surface electromyography (SEMG) is a measurement technique widely adopted for evaluating the activity of body surface muscles in a non-invasive way. Unfortunately the traditional SEMG measurement techniques are not suitable for the evaluation of the muscular activity and stress in rest positions, as during the optometric examinations. The main goal of this paper is to propose a new approach for the evaluation of muscular stress in patient wearing eyeglasses, based on the measurement and analysis for surface electromyographic signals, acquired in rest position for. Some experimental results, obtained with the proposed approach, are presented and discussed in the paper.