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Eukaryotic expression of Porcine BST-2 gene and identification of biological activity of BST-2

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4 Author(s)
Tinghong Zhang ; Coll. of Animal Sci. & Technol., Shandong Agric. Univ., Taian, China ; Zhenjie Zhang ; Xie Zhao ; Weishan Chang

BST-2 is a new kind of antivirus protein that is induced by IFN α/ β. The BST-2 gene fragment was amplified by RT-PCR from pig kidney cells (PK-15) and subcloned into the pLenti7.3/V5-TOPO vector and the positive clone was filtrated. The positive plasmids were transfected into the HEK293T cells. The expressed protein was analyzed by SDS-PAGE. Vesicular stomatitis virus (VSV)-CEF restrain experiment and Porcine reproductive and respiratory syndrome virus (PRRSV) intervence experiment were carried out. Results showed that the sequence of BST-2 gene amplified by RT-PCR was the same as the sequence in gene map of GenBank and its protein molecular weight was 23 kD, which was the same as the fusion protein V5 BST-2, and the recombinant BST-2 has obvious antiviral activity.

Published in:

Remote Sensing, Environment and Transportation Engineering (RSETE), 2011 International Conference on

Date of Conference:

24-26 June 2011