Cart (Loading....) | Create Account
Close category search window
 

Prokaryotic expression of the antigenic site B/C of S gene of porcine transmissible gastroenteritis virus and preparation of its monoclonal antibodies

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$31 $13
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

5 Author(s)
Xiaohua Nie ; Defeng CUI Lab. of Veterinary Microbiol., Beijing Univ. of Agric. Beijing, Beijing, China ; Huanrong Li ; Ran Gao ; Wang Xin
more authors

In this study, the antigenic site B/C of S gene of transmissible gastroenteritis virus (TGEV) was cloned into prokaryotic expression vector pET-28a and the recombinant plasmid was transferred into E.coli BL21 (DE3). The fusion protein was expressed after induction with IPTG. The SDS PAGE purified protein was used as immunogen. Six-week-old BALB/c mice were immunized three times with recombinant protein. The hybridoma cell strain, designated 5G, steadily secreting monoclonal antibodies (McAbs) against the protein of site B/C, was obtained by hybridoma technique. It belonged to IgM subclass and could recognize the site B/C recombinant protein of S gene of TGEV. It was confirmed that the McAbs only could react with TGEV using indirect immunofluorescence assay (IFA) and indirect ELISA, indicating the McAbs had good specificity.

Published in:

Remote Sensing, Environment and Transportation Engineering (RSETE), 2011 International Conference on

Date of Conference:

24-26 June 2011

Need Help?


IEEE Advancing Technology for Humanity About IEEE Xplore | Contact | Help | Terms of Use | Nondiscrimination Policy | Site Map | Privacy & Opting Out of Cookies

A not-for-profit organization, IEEE is the world's largest professional association for the advancement of technology.
© Copyright 2014 IEEE - All rights reserved. Use of this web site signifies your agreement to the terms and conditions.