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Ozone (O3) is a radiatively active trace gas that plies important role in atmosphere heating rates because of its good ability to absorb the infrared radiation and occur both at the ground level and naturally in the earth's upper atmosphere. Results from the analysis of the retrieved monthly (AIRX3STM) 1°×1° spatial resolution Atmosphere Infrared Sounder (AIRS) data were utilized to analyze the distribution of O3 column burden in Peninsular Malaysia for the period 2003-2009. AIRS is one of the several instruments onboard the Earth Observing System (EOS), onboard NASA's Aqua Satellite, launched on May 4, 2002. The analysis of O3 above five dispersed stations in study area shows the seasonal variation in the O3 values fluctuated considerably between wet and dry periods, and O3 values strongly correlated with weather conditions. The lowest O3 observed during rainy months, low temperature and low sunshine hours, vice versa. The highest O3 values occurred over Industrial and congested urban zones. The monthly O3 maps were generated, to study O3 distribution over peninsular Malaysia for 2009, using Kriging Interpolation technique. The AIRS data and the Satellite measurements are able to measure the increase of the atmosphere O3 concentrations over different regions.