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So far there is not an effective method to prevent the rapid spread of harmful microorganisms in air space or on the surface of objects. In this paper, a physical method based on strong electric-field discharge was used to produce hydroxyl radicals (·OH) for biological decontamination and sterilization in air space. Results show that cells of Staphylococcus aureus, Escherichia coli, and Yeast were rapidly killed by the ·OH fogdrops with a concentration of 0.6 mg/L and spray density of 22 μL/m2 within 4 s. Cell morphological changes were also observed through the microscope. Most of the cells were disrupted and lysed into small debris when being exposed to a killing dosage of ·OH radicals.