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Here, we investigate the relationship between scaffold topology and cell growth of neural cells on 3D scaffolds fabricated using Direct fs Laser Writing (DLW) of a polylactide-based material. DLW has been demonstrated as a technology for the fabrication of 3D structures with high resolution . The technique is based on the phenomenon of multi-photon polymerization. When the beam of an ultrafast infrared laser is tightly focused into the volume of a photosensitive material, the polymerization process can be initiated by nonlinear absorption within the focal volume. By moving the laser focus three-dimensionally through the photosensitive material, 3D structures can be fabricated. Recently, we explored DLW for use in the construction of biodegradable scaffolds using a poly-caprolactone based photopolymer.