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In this paper we describe an alternative variant dynamic Pickup and Delivery strategy of Vehicle Routing Problem (DVRP) which arises when new customers appear in the tours after starting visit. We propose an Artificial Ant Colony based on 2-Opt local search (AAC-2-Opt) to solve the DVRP. We demonstrate the effectiveness of our approach by comparing its results with those of existing methods in literature on the same tests problems. The proposed algorithm can efficiently optimize the routing problem and provide highly competitive solutions.