By Topic

Enhanced gene transfection using ultrasound and Vevo Micromarker® microbubbles: Microbubbles-assisted ultrasound and gene delivery

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$33 $13
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

5 Author(s)
Julien Piron ; INSERM U930 CNRS ERL 3106, B1A, CHU Bretonneau, 2 bd Tonnellé, 37044 Tours Cedex 9, France ; Jean-Michel Escoffre ; Kadija Kaddur ; Anthony Novell
more authors

Contrast agents for ultrasound imaging, composed of tiny gas microbubbles, have become a reality in clinical routine. Recent experimental studies showed that the combination of ultrasound with contrast agent microbubbles increase membrane permeability in a process known as sonoporation. This effect is thought to allow foreign molecules such as therapeutic genes to enter into the cells. The transfection level and efficiency have been shown to depend on the type of microbubbles. In that context, we performed a comparative study using 3 types of microbubbles, i.e. BR14®, SonoVue® and Vevo Micromarker® microbubbles. The transfection level and the cell viability of U-87 MG glioblastoma cells were measured. The results showed that the transfection level achieved with Vevo Micromarker® microbubbles is higher than BR14® and SonoVue® microbubbles with a comparable cell viability. The transfection rate obtained with Vevo Micromarker microbubbles reached approximately 70%. These results were correlated with the fact that Vevo Micromarker® microbubbles exhibited the lowest attenuation coefficient than BR14® and SonoVue® microbubbles at the insonation frequency.

Published in:

2010 IEEE International Ultrasonics Symposium

Date of Conference:

11-14 Oct. 2010