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Electrical Access to Lipid Bilayer Membrane Microchambers for Transmembrane Analysis

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5 Author(s)
Osaki, T. ; Lab. for Integrated Micro-Mechatron. Syst., Univ. of Tokyo, Tokyo, Japan ; Watanabe, Y. ; Kawano, R. ; Sasaki, H.
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This letter describes the integration of an electrical connection onto a lipid bilayer membrane microchip for the electrical detection/regulation of transmembrane phenomena together with fluorescence monitoring. Arrayed microchambers with a volume of 3 pL were fabricated within a poly(dimethyl siloxane) microfluidic device, and electrical access was achieved by designing channels adjacent to the chambers that were bridged by gold patterns on a glass substrate. We succeeded in the formation of a lipid bilayer membrane at the mouth of the chamber and applied electrical potential to the membrane with the integrated component. We confirmed the reconstitution of a transmembrane nanopore protein into the formed lipid bilayer membrane by signal monitoring with the electrical connection.

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Microelectromechanical Systems, Journal of  (Volume:20 ,  Issue: 4 )