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To lay foundation for the simulation of ECG algorithm and provide a signal source for studying the ECG monitoring system with the method to read the format of Classic ECG database and design a Physiological Signal Management and Playback System. First, to build a hardware platform with high-performance Personal Computer and digital-analog converter. Then, to establish ECG data functional unit in MFC class library, which is based on the Visual C++6.0 platform. Signal source can be controlled effectively as the output of ECG data and playback is synchronous. The interception, storage and playback of any section of ECG waveform have been achieved. The performance of the system stability and data accuracy will finally provide signal sources for simulation of ECG and development of instrumentation.