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The problems concerning multi-mineral source, multi-ore-dressing plant, multi-mineral of complex mining and ore-dressing systems in regional mine emerge gradually, with resources changes in new areas, such as an unreasonable overall layout of production, overlap and duplicated construction. From the perspective of complex systems theory, this article used the theories and methods of system science, computer science, mathematics, economics and so on, and also built the global optimization matching model. We study the global optimization of mining and ore-dressing systems of regional mine using Genetic Algorithm, that providing a theoretical basis for the realization of organic connection of complex systems in the region, overall best match and scientific and orderly exploitation of mineral resources etc. Thus, the level of development and utilization of mineral resources and economic benefits of regional mine have been increased.