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Nanostructured nickel oxide-chitosan film for application to cholesterol sensor

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4 Author(s)
Singh, Jay ; Department of Science and Technology Centre on Biomolecular Electronics Biomedical Instrumentation Section, National Physical Laboratory, Dr. K.S. Krishnan Marg, New Delhi 110012, India ; Kalita, Prasanta ; Singh, Jay ; Malhotra, B.D.

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Cholesterol oxidase (ChOx) physisorbed onto NiO nanoparticles (nNiO, 22 nm)— chitosan (CHIT) film prepared using coprecipitation method has been characterized using x-ray diffraction, Fourier transform infrared, scanning electron microscopy, cyclic voltammetry, and electrochemical impedance spectroscopy techniques. The results of electrochemical response studies conducted on ChOx/nan-NiO-CHIT/ITO bioelectrode show linearity of 10–400 mg/dl, detection limit of 43.4 mg/dl, sensitivity of 0.808 μA/(mgdlcm2), fast response time of 15 s, and shelf-life of about 10 weeks. The low value of Michaelis–Menten constant (Km) obtained as 0.67 mM indicates high affinity of ChOx toward the substrate.

Published in:

Applied Physics Letters  (Volume:98 ,  Issue: 12 )

Date of Publication:

Mar 2011

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