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The aim of this paper is to discuss the design and evaluation process of software for the analysis of ECG signal in a Holter system. The software was developed with RAD Studio 2009 using the Object Pascal language. The complete design and programming process was oriented to obtain different classes that encapsulate the needed functionality and combine them to obtain the final application. The analysis process was organized in different steps: acquisition of the 3 channels of ECG and other recording information from the record device, filtration of ECG signal to remove movement and electrodes noises, detection and classification of QRS, detection of rhythm events and pacemaker malfunction, search of ST deviation episodes, HRV and spatial QT dispersion studies. All the processes were executed in this order and finalize with the compression and storage of the results.