By Topic

Number of kinesin molecules involved in a bead transport measured by microfluidics and mechanical modeling

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$31 $13
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

5 Author(s)

In contrast to optical tweezers methods, we established a method to evaluate the adhesion force between a bead carried by multiple kinesins and a microtubule. The method also enables us to estimate the number of kinesins involved in the bead transport. Torque Γ and drag force FD applied by the shearing flow were derived theoretically by the flow rate necessary to break kinesin-microtubule bindings in the bead detachment assay. Based on mechanical modeling, the adhesion force, FAdh, was calculated as 362.9 pN without ATP and 31.3 pN with 1 mM ATP. Furthermore, we found out 5.5-11.9 kinesins are cooperatively working to carry the bead, which is consistent with other results. Our method is advantageous in analyzing multi-kinesin transport system reconstructed in microfluidic systems.

Published in:

Micro Electro Mechanical Systems (MEMS), 2011 IEEE 24th International Conference on

Date of Conference:

23-27 Jan. 2011