Label-free adhesion-based cell sorter using optimized oblique grooves for early cancer detection

We report development of a label-free adhesion-based cell sorter for early cancer detection. The sorting method is based on specific adhesion between target cells and antibody immobilized on oblique grooves of the channel wall. By optimizing the groove dimensions, lateral cell displacement twice as large as our earlier report has been obtained. Continuous lateral separation of HeLa cancer cells from mouse whole blood has been demonstrated.