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Pairing single adherent cells in the dynamic microarray

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4 Author(s)
Teshima, T. ; Inst. of Ind. & Sci., Univ. of Tokyo, Tokyo, Japan ; Onoe, H. ; Kuribayashi-Shigetomi, K. ; Takeuchi, S.

This paper describes a dynamic microarray system for pairing adherent cells at single cell level. By introducing single cells cultured on mobile Parylene microplates into the system, we achieved pairing of single cells maintaining their physiological properties. By taking advantage of processibility of Parylene, we changed the shape and fabricated microgrooves on the plate surface. These modifications enabled us to control cell stretch and morphology on the micro-sized plates and bring single cells into contact easily. We expect this method is applicable to the analysis of a variety of cell-cell communication between two single adherent cells.

Published in:

Micro Electro Mechanical Systems (MEMS), 2011 IEEE 24th International Conference on

Date of Conference:

23-27 Jan. 2011

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