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This paper introduces a radioactive source tracer anti-theft system, which mainly consists of three parts-Detection probe, Tracker and PC. It is based on RFID, GSM and GPS. The system can realize non-contact information acquisition processing for the stolen radioactive source. If there is unauthorized or illegal movement of radioactive sources, GPS/GSM system which is installed in the radioactive source tracer anti-theft system starts work automatically, implement monitoring and tracking function, then regularly to send alarm message to the specified phone number and PC, and displays the location of the radioactive source, achieves automatic monitoring of radioactive source management objectives.