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The removal effect of loess-microorganism system on oil pollution treatment was studied by static simulation laboratory experiments in this paper. The influencing factors such as nutrient substance, temperature, oxidizer and pH were analyzed. The optimal ecological condition was determined through orthogonal experiment. The results show that the best ecological condition of Loess-Microorganism System is as follow: nutrient proportion (C:N:P) is 500:6:1, temperature is 35°C, the accumulation dosage of H2O2 is 12mg/L, and pH is 7.