Scheduled System Maintenance:
On May 6th, single article purchases and IEEE account management will be unavailable from 8:00 AM - 5:00 PM ET (12:00 - 21:00 UTC). We apologize for the inconvenience.
By Topic

Model-based sequential base calling for Illumina sequencing

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$31 $13
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

3 Author(s)
Das, S. ; Electr. & Comput. Eng. Dept., Univ. of Texas at Austin, Austin, TX, USA ; Vikalo, H. ; Hassibi, A.

In this paper, we study the efficacy of a model-based base-calling approach for Illumina's sequencing platforms. In particular, we investigate Genome Analyzer I reads and provide a detailed biochemical model of the sequencing process, incorporating various non-idealities evident in such systems. Parameters of the model are estimated via a supervised learning based on the particle swarm optimization technique. A computationally efficient sequential decoding method is proposed for base-calling. It is demonstrated that the performance of the proposed approach is comparable to Illumina's base-calling method.

Published in:

Genomic Signal Processing and Statistics (GENSIPS), 2010 IEEE International Workshop on

Date of Conference:

10-12 Nov. 2010