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Monitoring laboratory equipment record is important to ensure every item is always in place. Generally, in and out equipment is handled manually by technician by writing down the equipment information, including time and date in equipment circulation form. To automate the process, Radio Frequency Identification (RFID) is one of the most practical and applicable in real implementation in-line with the nature where most of the systems are made computerized. In this paper, a solution has been provided for the problem encountered in laboratory equipment monitoring system using RFID technology. This project consist four main parts: the tag, tag reader, system development and networking system. The RFID tag is tagged on the laboratory equipment where the tag contains laboratory equipment information and RFID reader is located at the door of each laboratory room. This monitoring system enables the head of laboratory and technician to monitor in-out equipment in actual environment and also increase the efficiency in managing equipment in the laboratory. Benefits of the system include enhancement of the safety of University asset and reduce losses of assets and enhancement of the laboratory inventory control of equipment.