By Topic

Optimization of surface plasmon resonance glucose detection based on D-galactose/D-glucose protein amine coupling method

Sign In

Cookies must be enabled to login.After enabling cookies , please use refresh or reload or ctrl+f5 on the browser for the login options.

Formats Non-Member Member
$31 $13
Learn how you can qualify for the best price for this item!
Become an IEEE Member or Subscribe to
IEEE Xplore for exclusive pricing!
close button

puzzle piece

IEEE membership options for an individual and IEEE Xplore subscriptions for an organization offer the most affordable access to essential journal articles, conference papers, standards, eBooks, and eLearning courses.

Learn more about:

IEEE membership

IEEE Xplore subscriptions

4 Author(s)
Peng Wu ; State Key Lab. of Precision Meas. Technol. & Instrum., Tianjin Univ., Tianjin, China ; Dachao Li ; Jingxin Zhang ; Kexin Xu

Measuring the glucose concentration of interstitial fluid in human body is an effectively way to control and treat diabetes. A surface plasmon resonance (SPR) system based on a miniature integrated SPR sensor is presented in this paper to measure the glucose concentration in interstitial fluid. D-galactose/D-glucose Binding Protein (GGBP) which can specifically absorb glucose is immobilized onto the SPR sensor surface by amine coupling method for higher sensitivity. The experiment result is affected by various factors, such as baseline, flow velocity, mass transfer effect, temperature, bubbles. In this paper these factors are studied systemically by experiments and some solutions are proposed accordingly: (1) Dynamic baseline can provide the best baseline location during the measurement. (2) Flow velocity of 10uL/min-30uL/min is supposed to be chosen. (3) The density of protein immobilized on the sensor surface should maintain lower to avoid the mass transfer effect. (4) Higher response is got when the temperature is between 26°C and 30°C and it is important to keep temperature constant during the experiment. (5) Samples should be degassed and filtered before the experiment and make sure there is no air in the pipeline. Through optimizing the experiment conditions, the sensitivity and stability of measurement are improved.

Published in:

Advances in Optoelectronics and Micro/Nano-Optics (AOM), 2010 OSA-IEEE-COS

Date of Conference:

3-6 Dec. 2010