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One of the most common cardiovascular diseases is Myocardial Ischemia (MI). The aim of this study is improving the diagnosis level of Ischemic Beat detection from ECG signals which is important in ischemic episode detection process. This improvement is based on appropriate feature extraction via Multi resolution Wavelet analysis and proper classifier selection. The approach starts with signal preprocessing, Afterwards efficacious morphologic features which are useful in ischemia detection are extracted by wavelet analysis. In the third stage subtractive clustering is performed for clustering. Finally probabilistic neural networks are used as a classifier. The proposed algorithm is evaluated on the European Society of Cardiology (ESC) ST-T database and reported 96.67% sensitivity and 89.18% specificity.