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Gold (Au) nanoparticles were fabricated on Chrome (Cr) thin film to glass substrate by slow-rate of e-beam evaporation method. Gold nanoparticles were identified by using scanning electron microscopy, atomic force microscopy and energy dispersive X-ray spectroscopy measurements. Au and Cr-peaks were confirmed by single crystal X-ray diffraction measurements. Energy dispersive X-ray and UV-spectroscopy measurements revealed that the elemental compositions, absorbance, transmission and reflectance of Au/Cr thin-films deposited on glass substrate, respectively. Au layers with thickness of about 1, 5 and 10 nm were deposited by e-beam evaporation method on unheated glass substrates coated with a Cr layer about 30 nm thick. Owing to the surface plasmon resonance effect, gold nanoparticles on Cr/glass have strong extinction absorbance from 400 to 600 nm wavelength regions for 5 and 10 nm thick of gold thin films deposited on glass substrates.