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Sensing of antibodies secreted by microfluidically trapped cells via extraordinary optical transmission through nanohole arrays

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11 Author(s)
Romanuik, S.F. ; ENSC, Simon Fraser Univ., Burnaby, BC, Canada ; Grist, S.M. ; Gray, B.L. ; Hohertz, D.
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This work presents a multi-component chip that: (i) traps settling cells into poly(dimethyl siloxane) microwells; and (ii) senses the binding of antibodies to antigen immobilized onto a gold surface via the surface plasmon resonance shifts of milled nanohole arrays. We have previously detected the 17/9 antibodies secreted by ~200 hybridoma cells/trap. This work demonstrates our system's current capabilities and limitations via a follow-up experiment using a reduced ~5-20 hybridoma cells/trap density.

Published in:

Sensors, 2010 IEEE

Date of Conference:

1-4 Nov. 2010