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Fast cell immobilization by using non-immunological method for cell based biosensor

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8 Author(s)
Jihwang Park ; Interdisciplinary Human Biotechnology, Korea Institute of Science and Technology Europe GmbH, Saarbrücken, Germany ; Michael Müller ; Eunji Jang ; Jungtae Kim
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This paper reports a concept of a micro cell analysis system for fast cell immobilization by using a non immunological method which is composed of a cell/drug inlet, a sheath flow inlet, a micro chamber and a outlet to biosensor/waste. Micro cell wells were located in the micro chamber. To define optimized parameters of micro cell analysis system, cell adhesion experiment using Hela at 0.5×106, 1.0×106 or 2.0×106 cells/ml in micro wells with 10μm depth and 25μm, 50μm and 100μm size respectively was performed. Different tendencies of cell adhesion between 0.5×106 cells/ml and 1.0×106 cells/ml concentration were not detected. The highest concentration gave the best performance of cell immobilization, even though cell congregation appeared.

Published in:

Sensors, 2010 IEEE

Date of Conference:

1-4 Nov. 2010