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Generation of a quasi-optical single sideband (OSSB) signal for a radio-over-fiber system utilizing the coherent population oscillation effects in a nonlinear semiconductor optical amplifier and transmission over a single-mode fiber (SMF) are investigated. The quasi-OSSB signal generator consists of an electroabsorption modulator and a nonlinear semiconductor optical amplifier. Characteristics of generated quasi-OSSB signals having different radio-frequency (RF) waves (1-50 GHz) transmitted over SMFs of different lengths (4-21 km) were investigated. Bit-error-rate performance of quasi-OSSB signals having 155-Mb/s differential phase-shift keying (DPSK) data transmitted over 0- to 55-km-long SMFs was also evaluated.