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High-resolution wide-field standing-wave surface plasmon resonance fluorescence microscopy with optical vortices

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4 Author(s)
Tan, P.S. ; School of Electrical and Electronic Engineering, Nanyang Technological University, Nanyang Avenue, 639798 Singapore ; Yuan, X.-C. ; Yuan, G.H. ; Wang, Q.

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A technique for high-resolution wide-field fluorescence microscopy by using standing surface plasmon waves induced by optical vortices (OVs) is proposed, aiming at harnessing its unique dynamic properties to sequentially illuminate specimen with different phase-shifted interference excitation field. Topological charges of the OV are employed to modulate phase-shifting of the interference pattern. Numerical studies and experimental results reveal an achievement of 0.25λ optical resolution, a factor of twofold improvement compared with the standard total-internal-reflection fluorescence microscopy.

Published in:

Applied Physics Letters  (Volume:97 ,  Issue: 24 )

Date of Publication:

Dec 2010

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