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Automatic measurement system for biological applications based on pH using ISFET sensor probe

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4 Author(s)
Yamada, A. ; Dept. of Mech. Syst. Eng., Hiroshima Inst. of Technol., Hiroshima, Japan ; Mohri, S. ; Nakamura, M. ; Naruse, K.

Throughput in the cell response analysis is expected to progress in medicine and biology. In this paper, we construct a method for the measurement of cell activity that is measured by the pH fluctuation of the solution around the cell. To improve throughput of the measurements, first we constructed an automatic pH measurement system using ion-sensitive field-effect transistor (ISFET), and the system composed of 96-well microplate, pump, XYZ stage, and PC. The basic data of the cell activity was taken from human embryonic kidney 293 (HEK 293) cells by the use of flow-through type measuring chamber.

Published in:
Micro-NanoMechatronics and Human Science (MHS), 2010 International Symposium on

Date of Conference: 7-10 Nov. 2010

Page(s):
19 - 24
Print ISBN:
978-1-4244-7995-5
INSPEC Accession Number:
11699277
Conference Location :
Nagoya
Digital Object Identifier :
10.1109/MHS.2010.5669583
Product Type:
Conference Publications

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