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The increasing protein sequences from the genome project require theoretical methods to predict transmembrane helical segments (TMHs). In this paper, a method based on discrete wavelet transform (DWT) has been developed to predict the number and location of TMHs in membrane proteins. PDB coded as 1F88 is chosen as an example to describe the prediction process with this method. One group of test data sets that contain total 25 protein sequences were utilized to access the effect of this method. Compared with main prediction results of five common prediction methods, the obtained results indicate that the proposed method has higher prediction accuracy.