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Nano gold-L-alanine modified electrode was prepared and its application to the electrochemical determinatino of formaldehyde in alkaline medium was studied by cyclic voltammetry. The experimental parameters were optimized, and a voltammetric method for determining formaldehyde was developed. The characteristics of CVs showed the electrocatalytic oxidation peak current of formaldehyde is proportional to the concentration of formaldehyde over the range of 1.0×10-5 ~1.6×10-2mol/L with the detection limit of 6.3×10-7mol/L in alkaline solution (r = 0.9975). The recovery is 98% - 103%. So this nano Au-L-alanine modified electrode can be developed as a rapid, sensitive electrochemical detection method for formaldehyde.