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Recently, the materials scientists invented that the dye functionalized MWCNT found applications in photodynamic therapy, due to the apoptosis of targeted cancer cells by the coupled effect of photothermal as well as photodynamic effects. By grouping these ideas, a new type of material was synthesized. In the present investigation, Eosin Y, a xanthenes type dye was functionalized with MWCNT via ethylene diamine (EDA) as a coupling agent. The Eosin Y functionalized MWCNT was characterized by the Fourier transform infrared (FTIR) spectroscopy, UV-Visible spectroscopy, photoluminescence (PL) spectroscopy, X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM) and transmission electron microscopy (TEM) techniques.