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In this letter, we theoretically propose a biosensor based on a two-dimensional left-handed photonic crystal coupled to an M-shaped resonator. The sensor is optimized to operate in an aqueous environment. By controlling the radius of the air cylinder defect, a strong interaction between light and biomolecules in aqueous solution is achieved. We demonstrate numerically that by confining the biomolecular into the region of interest, a high sensitivity is reached. It is shown that shifts in the peak wavelength values are strongly related to the refractive index changes.