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To eliminate interference within electrocardiogram signal (ECS) recorded under complicated Power Line (PL) circumstances, a novel algorithm is proposed. ECS is divided into two parts, linear segment and the nonlinear one. A new linear segment determination rule is presented, by which linear and non-linear segment can be distinguished within single PL period by using the symmetry of sinusoidal wave. Correlation algorithm is used to calculate the amplitude and initial phase of Power Line Interference (PLI). Performance of the proposed algorithm is compared with and Subtraction Procedure (SP) on MATLAB platform. The presented algorithm evidently improves PLI evaluation update times, and avoids boundary distortion, and efficiently eliminates PLI. Experiment results show that the new algorithm is superior in filtering amplitude-changed PLI.